Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia
Wenqi Wang, Helen Owen, Rebecca J Traub, Leigh Cuttell, Tawin Inpankaew, Helle Bielefeldt-Ohmann
Veterinary Parasitology | ELSEVIER SCIENCE BV | Published : 2014
Blastocystis, an intestinal protist commonly found in humans and animals worldwide, has been implicated by some as a causative agent in irritable bowel syndrome in humans. In pigs, infection with Blastocystis is commonly reported, with most pigs shown to harbour subtypes (ST) 1 or 5, suggesting that these animals are potentially natural hosts for Blastocystis. Although ST5 is considered rare in humans, it has been reported to be a potential zoonosis from pigs in rural China. To test these hypotheses, we conducted molecular analysis of faecal samples from pigs and in-contact humans from commercial intensive piggeries in Southeast Queensland (SEQ), Australia, and a village in rural Cambodia. T..View full abstract
This project has been supported by the William Peter Richards Trust Award Grant (WW) and The University of Queensland New Staff Start-up Grant (HO). Special thanks to Paul Noone, piggery staff, staff in the School of Veterinary Science especially Dr Kit Parke and the UQVeterinary Clinical Studies Centre for their assistance in sample collection and processing. The Cambodian samples were collected as part of Dr. Tawin Inpankaew's PhD project that is funded by the University of Copenhagen, Denmark. We would also like to acknowledge staff of The National Center for Parasitology, Entomology and Malaria Control, Cambodia, Mr Wissanuwat Chimnoi and Ms Supanun Boonchob, staff of The faculty of Veterinary Medicine, Kasetsart University, Thailand, Dr Chhay Somany, deputy director of the provincial health department at Preah Vihear province and all the participants for their assistance in sample collection from Dong village in Cambodia. Lastly, many thanks to Dr. Christen Rune Stensvold for kindly giving us access to the STS 1, 2 and 3 primers that he designed.