Stable isotope marking of otoliths during vaccination: a novel method for mass-marking fish
Fletcher Warren-Myers, Tim Dempster, Per Gunnar Fjelldal, Tom Hansen, Arne J Jensen, Stephen E Swearer
Aquaculture Environment Interactions | INTER-RESEARCH | Published : 2014
Tagging or marking of fishes enables the collection of population-based information for ecological research, yet few techniques enable 100% mark detection success. We tested a new mass-marking technique: otolith marking with enriched stable isotopes delivered during vaccination. Atlantic salmon (Salmo salar) parr were injected in either the abdominal cavity or muscle with a combination of enriched Ba, Sr and Mg, using 1 of 3 carrier solutions (water, vaccine, vaccine mimic). Laser ablation inductively coupled plasma mass spectrometry of the otoliths indicated that Ba and Sr isotope enrichment treatments achieved 100% mark success, with 0 to 34% success for Mg, compared to experimental ..View full abstract
Awarded by Norwegian Fisheries and Aquaculture Research Fund
We thank Lise Dyrhovden and the technical staff from the Matre Aquaculture Research Station and Ingebrigt Uglem (NINA) for facilitating access to the Norwegian Institute for Nature Research Atlantic salmon archive. Alan Greig from the School of Earth Sciences, University of Melbourne, assisted with LA-ICP-MS analyses. Funding was provided by the Norwegian Fisheries and Aquaculture Research Fund (project #900710).