Conference Proceedings

Analyzing hematopoietic stem cell homing, lodgment, and engraftment to better understand the bone marrow niche

Shen Y Heazlewood, Ana Oteiza, Huimin Cao, Susan K Nilsson, JM Rice (ed.)

Annals of the New York Academy of Sciences | BLACKWELL SCIENCE PUBL | Published : 2014

Abstract

The existence of a bone marrow (BM) niche--the location in which hematopoietic stem cells (HSCs) reside--was proposed more than 30 years ago. Recent data suggest that the interaction of HSCs with cellular and extracellular components within the BM is critical for HSC regulation. The tracking of immunofluorescently labeled, prospectively isolated HSCs to and within the BM cavity allows the assessment of the regulatory processes involved in (1) homing, which involves transendothelial migration into the BM; (2) lodgment, including transmarrow migration through the extravascular space; and (3) BM reconstitution. Together, such analyses provide a better understanding of the cellular and extracell..

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University of Melbourne Researchers