Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling
Akram Ghantous, Richard Saffery, Marie-Pierre Cros, Anne-Louise Ponsonby, Steven Hirschfeld, Carol Kasten, Terence Dwyer, Zdenko Herceg, Hector Hernandez-Vargas
BMC Biotechnology | BMC | Published : 2014
BACKGROUND: Neonatal dried blood spots (DBS) represent an inexpensive method for long-term biobanking worldwide and are considered gold mines for research for several human diseases, including those of metabolic, infectious, genetic and epigenetic origin. However, the utility of DBS is restricted by the limited amount and quality of extractable biomolecules (including DNA), especially for genome wide profiling. Degradation of DNA in DBS often occurs during storage and extraction. Moreover, amplifying small quantities of DNA often leads to a bias in subsequent data, particularly in methylome profiles. Thus it is important to develop methodologies that maximize both the yield and quality of DN..View full abstract
Awarded by NICHD
We thank Dr. Florence Le Calvez-Kelm and Mr. Geoffroy Durand for helping in the HM450 experimental process and bioanalyzer measurements. This work was partially supported by the Association pour le Recherche Contre le Cancer (l'ARC). AG is supported by the IARC Postdoctoral Fellowship and Marie Curie Actions-People-COFUND. We thank the International Childhood Cancer Cohort Consortium for contributing to the coordination between participating cohorts.The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Children's Study, National Institutes of Health, or U.S. Department of Health and Human Services. Supported in part by NICHD Contracts HHSN275200503414C, HHSN275200503411C, HHSN275200603416C, HHSN275200503415C, HHSN275200503413C, HHSN275200503410C, HHSN275200503396C, HHSN275201000121U, and HHSN275200900010C.