Genetic Diversity and Virulence Potential of Shiga Toxin-Producing Escherichia coli O113:H21 Strains Isolated from Clinical, Environmental, and Food Sources
Peter CH Feng, Sabine Delannoy, David W Lacher, Luis Fernando dos Santos, Lothar Beutin, Patrick Fach, Marta Rivas, Elizabeth L Hartland, Adrienne W Paton, Beatriz EC Guth
Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2014
Shiga toxin-producing Escherichia coli strains of serotype O113:H21 have caused severe human diseases, but they are unusual in that they do not produce adherence factors coded by the locus of enterocyte effacement. Here, a PCR microarray was used to characterize 65 O113:H21 strains isolated from the environment, food, and clinical infections from various countries. In comparison to the pathogenic strains that were implicated in hemolytic-uremic syndrome in Australia, there were no clear differences between the pathogens and the environmental strains with respect to the 41 genetic markers tested. Furthermore, all of the strains carried only Shiga toxin subtypes associated with human infection..View full abstract
Awarded by French Joint Ministerial Program of R&D against CBRNE Risks
The microarray and CRISPR assays were developed with funding from the French Joint Ministerial Program of R&D against CBRNE Risks (grant C17609-2). The characterizations of the Brazilian strains were done with a past grant from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of Brazil.