Influenza A virus and TLR7 activation potentiate NOX2 oxidase-dependent ROS production in macrophages
EE To, BRS Broughton, KS Hendricks, R Vlahos, S Selemidis
FREE RADICAL RESEARCH | TAYLOR & FRANCIS LTD | Published : 2014
Influenza A virus infects resident alveolar macrophages in the respiratory tract resulting in Toll like receptor 7 (TLR7) activation that triggers an inflammatory response to resolve the infection. Macrophages are also major sources of reactive oxygen species (ROS) via the NOX2-containing NADPH oxidase. Although ROS are crucial for pathogen clearance, in response to influenza A virus, ROS are touted as being culprit mediators of the lung tissue injury. The aim of the present study was to determine whether influenza A virus infection and TLR7 activation of macrophages, results in alterations in their ROS production. Here we demonstrate using immunofluorescence that influenza A virus (Hong Kon..View full abstract
Related Projects (1)
Awarded by Australian Research Council
Awarded by National Health and Medical Research Council of Australia
The authors wish to thank the Australian Research Council for fellowship and funding support (FT120100876) for Dr Stavros Selemidis and the National Health and Medical Research Council of Australia for funding support (APP1027112). Also the authors wish to thank Dr John Stambas for providing stocks of X-31 influenza A virus.