Journal article

Effect of Reduced Retinal VLC-PUFA on Rod and Cone Photoreceptors

Lea D Bennett, Richard S Brush, Michael Chan, Todd A Lydic, Kristen Reese, Gavin E Reid, Julia V Busik, Michael H Elliott, Robert E Anderson

Investigative Ophthalmology and Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2014

Abstract

PURPOSE: Autosomal dominant Stargardt-like macular dystrophy (STGD3) is a juvenile-onset disease that is caused by mutations in Elovl4 (elongation of very long fatty acids-4). The Elovl4 catalyzes the first step in the conversion of C24 and longer fatty acids (FAs) to very long-chain FAs (VLC-FAs, ≥C26). Photoreceptors are particularly rich in VLC polyunsaturated FAs (VLC-PUFA). To explore the role of VLC-PUFAs in photoreceptors, we conditionally deleted Elovl4 in the mouse retina. METHODS: Proteins were analyzed by Western blotting and lipids by gas chromatography (GC)-mass spectrometry, GC-flame ionization detection, and tandem mass spectrometry. Retina function was assessed by electroreti..

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University of Melbourne Researchers

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Awarded by NIH



Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES


Awarded by NATIONAL EYE INSTITUTE


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

Supported by NIH Grants EY00871, EY04149, P30EY021725, and P20RR017703 (REA); and EY019494 (MHE); and by the Foundation Fighting Blindness (REA); Research to Prevent Blindness (Departmental); and Grants GM103508 (GER, JVB) and EY016077 (JVB, GER).