Journal article

Fragile TIM-4-expressing tissue resident macrophages are migratory and immunoregulatory

Thomas B Thornley, Zemin Fang, Savithri Balasubramanian, Rafael A Larocca, Weihua Gong, Shipra Gupta, Eva Csizmadia, Nicolas Degauque, Beom Seok Kim, Maria Koulmanda, Vijay K Kuchroo, Terry B Strom

JOURNAL OF CLINICAL INVESTIGATION | AMER SOC CLINICAL INVESTIGATION INC | Published : 2014

Abstract

Macrophages characterized as M2 and M2-like regulate immune responses associated with immune suppression and healing; however, the relationship of this macrophage subset to CD169+ tissue-resident macrophages and their contribution to shaping alloimmune responses is unknown. Here we identified a population of M2-like tissue-resident macrophages that express high levels of the phosphatidylserine receptor TIM-4 and CD169 (TIM-4hiCD169+). Labeling and tracking of TIM-4hiCD169+ macrophages in mice revealed that this population is a major subset of tissue-resident macrophages, homes to draining LNs following oxidative stress, exhibits an immunoregulatory and hypostimulatory phenotype that is maint..

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University of Melbourne Researchers

Grants

Awarded by National Institute of Allergy and Infectious Disease


Awarded by Juvenile Diabetes Research Foundation


Awarded by National Institute of Diabetes and Digestive and Kidney Diseases


Awarded by National Heart, Lung, and Blood Institute


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Funding Acknowledgements

We thank Vasilis Toxavidis, John Tigges, Heidi Mariani, Sarah Schuett, Marissa Fahlberg, and Kaitlin Groglio for their expertise and assistance with flow cytometry and cell sorting. This work was funded by the National Institute of Allergy and Infectious Disease (grant 5P01A1073748, to T.B. Strom and V.K. Kuchroo), the Juvenile Diabetes Research Foundation (grant 3-2010-792, to T.B. Thornley), the National Institute of Diabetes and Digestive and Kidney Diseases (grant 5R01DK096138, to T.B. Strom, M. Koulmanda, and V.K. Kuchroo), and the National Heart, Lung, and Blood Institute (training grant T32HL007893, to T.B. Thornley).