A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis
Hayley J Newton, Lara J Kohler, Justin A McDonough, Morayma Temoche-Diaz, Emerson Crabill, Elizabeth L Hartland, Craig R Roy
PLoS Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2014
Coxiella burnetii is an intracellular pathogen that replicates in a lysosome-derived vacuole. The molecular mechanisms used by this bacterium to create a pathogen-occupied vacuole remain largely unknown. Here, we conducted a visual screen on an arrayed library of C. burnetii NMII transposon insertion mutants to identify genes required for biogenesis of a mature Coxiella-containing vacuole (CCV). Mutants defective in Dot/Icm secretion system function or the PmrAB regulatory system were incapable of intracellular replication. Several mutants with intracellular growth defects were found to have insertions in genes encoding effector proteins translocated into host cells by the Dot/Icm system. Th..View full abstract
Awarded by NIH
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
This work was supported by NIH Grant U54AI057158. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.