A New Method To Determine In Vivo Interactomes Reveals Binding of the Legionella pneumophila Effector PieE to Multiple Rab GTPases
Aurelie Mousnier, Gunnar N Schroeder, Charlotte A Stoneham, Ernest C So, James A Garnett, Lu Yu, Steve J Matthews, Jyoti S Choudhary, Elizabeth L Hartland, Gad Frankel
mBio | AMER SOC MICROBIOLOGY | Published : 2014
UNLABELLED: Legionella pneumophila, the causative agent of Legionnaires' disease, uses the Dot/Icm type IV secretion system (T4SS) to translocate more than 300 effectors into host cells, where they subvert host cell signaling. The function and host cell targets of most effectors remain unknown. PieE is a 69-kDa Dot/Icm effector containing three coiled-coil (CC) regions and 2 transmembrane (TM) helices followed by a fourth CC region. Here, we report that PieE dimerized by an interaction between CC3 and CC4. We found that ectopically expressed PieE localized to the endoplasmic reticulum (ER) and induced the formation of organized smooth ER, while following infection PieE localized to the Legio..View full abstract
Awarded by Wellcome Trust
Awarded by Engineering and Physical Sciences Research Council
Awarded by Medical Research Council
This project was supported by grants from the Wellcome Trust and the Medical Research Council UK (G.F.). J.S.C. and L.Y. are supported by the Wellcome Trust (079643/Z/06/Z).