Journal article

Multiple ecto-nucleoside triphosphate diphosphohydrolases facilitate intracellular replication of Legionella pneumophila

Patrice Riedmaier, Fiona M Sansom, Trifina Sofian, Travis Beddoe, Ralf Schuelein, Hayley J Newton, Elizabeth L Hartland

Biochemical Journal | PORTLAND PRESS LTD | Published : 2014

Abstract

Legionella pneumophila is an opportunistic pathogen that replicates within alveolar macrophages resulting in the onset of severe atypical pneumonia. Previously we have identified Lpg1905, a eukaryotic-type ecto-NTPDase (nucleoside triphosphate diphosphohydrolase) from L. pneumophila that was required for optimal intracellular replication and virulence in a mouse lung infection model. In the present study, we characterized the activity of a second eukaryotic-type NTPDase, Lpg0971, from L. pneumophila. We observed that recombinant Lpg0971 hydrolysed only ATP and exhibited divalent cation preference for manganese (II) ions. Similar to lpg1905, an lpg0971 mutant carrying the plasmid pMIP was att..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by Australian Research Council (ARC)


Funding Acknowledgements

This work was supported by the Australian National Health and Medical Research Council (NHMRC) [grant number 606788 (to E.L.H.)] and Australian Research Council (ARC) [grant number DP120100805 (to TB. and EMS.)]. E.L.H. was supported by an ARC Future Fellowship. P.R. was supported by an Australian Postgraduate Award. T.B. was supported by Pfizer Australia Research fellowship. H.J.N. and F.M.S. were supported by an NHMRC Australian Training Award.