Journal article

H5N1-SeroDetect EIA and Rapid Test: a Novel Differential Diagnostic Assay for Serodiagnosis of H5N1 Infections and Surveillance

Surender Khurana, Pretty Sasono, Annette Fox, Van Kinh Nguyen, Quynh Mai Le, Quang Thai Pham, Tran Hien Nguyen, Thanh Liem Nguyen, Peter Horby, Hana Golding

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2011

Abstract

Continuing evolution of highly pathogenic (HP) H5N1 influenza viruses in wild birds with transmission to domestic poultry and humans poses a pandemic threat. There is an urgent need for a simple and rapid serological diagnostic assay which can differentiate between antibodies to seasonal and H5N1 strains and that could provide surveillance tools not dependent on virus isolation and nucleic acid technologies. Here we describe the establishment of H5N1 SeroDetect enzyme-linked immunosorbent assay (ELISA) and rapid test assays based on three peptides in HA2 (488-516), PB1-F2 (2-75), and M2e (2-24) that are highly conserved within H5N1 strains. These peptides were identified by antibody repertoi..

View full abstract

University of Melbourne Researchers