Journal article

Endothelial Transcriptome in Response to Pharmacological Methyltransferase Inhibition

Jun Okabe, Ana Z Fernandez, Mark Ziemann, Samuel T Keating, Aneta Balcerczyk, Assam El-Osta

ChemMedChem | WILEY-V C H VERLAG GMBH | Published : 2014

Abstract

The enzymatic activities of protein methyltransferases serve to write covalent modifications on histone and non-histone proteins in the control of gene transcription. Here, we describe gene expression changes in human endothelial cells caused by treatment with methyltransferase inhibitors 7,7'-carbonylbis (azanediyl) bis(4-hydroxynaphthalene-2 -sulfonic acid (AMI-1) and disodium-2-(2,4,5,7- tetrabromo-3-oxido-6-oxoxanthen-9-yl) benzoate trihydrate (AMI-5). Deep sequencing of mRNA indicated robust change on transcription following AMI-5 treatment compared with AMI-1. Functional annotation analysis revealed that both compounds suppress the expression of genes associated with translational regu..

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University of Melbourne Researchers

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Funding Acknowledgements

The authors would like to thank Antony Kaspi and Dr. Ross Lazarus (Baker IDI Heart & Diabetes Institute) for expert bioinformatics support, as well as Dr. Tom Karagiannis (Baker IDI Heart & Diabetes Institute) for technical advice. The authors acknowledge grant and fellowship support from the International Juvenile Diabetes Research Foundation (JDRF), the Diabetes Australia Research Trust (DART), the Australian National Health & Medical Research Council (NHMRC) and the Australian National Heart Foundation (NHF).