Endothelial Transcriptome in Response to Pharmacological Methyltransferase Inhibition
Jun Okabe, Ana Z Fernandez, Mark Ziemann, Samuel T Keating, Aneta Balcerczyk, Assam El-Osta
ChemMedChem | WILEY-V C H VERLAG GMBH | Published : 2014
The enzymatic activities of protein methyltransferases serve to write covalent modifications on histone and non-histone proteins in the control of gene transcription. Here, we describe gene expression changes in human endothelial cells caused by treatment with methyltransferase inhibitors 7,7'-carbonylbis (azanediyl) bis(4-hydroxynaphthalene-2 -sulfonic acid (AMI-1) and disodium-2-(2,4,5,7- tetrabromo-3-oxido-6-oxoxanthen-9-yl) benzoate trihydrate (AMI-5). Deep sequencing of mRNA indicated robust change on transcription following AMI-5 treatment compared with AMI-1. Functional annotation analysis revealed that both compounds suppress the expression of genes associated with translational regu..View full abstract
The authors would like to thank Antony Kaspi and Dr. Ross Lazarus (Baker IDI Heart & Diabetes Institute) for expert bioinformatics support, as well as Dr. Tom Karagiannis (Baker IDI Heart & Diabetes Institute) for technical advice. The authors acknowledge grant and fellowship support from the International Juvenile Diabetes Research Foundation (JDRF), the Diabetes Australia Research Trust (DART), the Australian National Health & Medical Research Council (NHMRC) and the Australian National Heart Foundation (NHF).