Differentiation of Stem Cells from Human Infrapatellar Fat Pad: Characterization of Cells Undergoing Chondrogenesis
Raed Felimban, Ken Ye, Kathy Traianedes, Claudia Di Bella, Jeremy Crook, Gordon G Wallace, Anita Quigley, Peter FM Choong, Damian E Myers
Tissue Engineering Part A | MARY ANN LIEBERT, INC | Published : 2014
Hyaline cartilage repair is a significant challenge in orthopedics and current techniques result in formation of fibrocartilage. Human infrapatellar fat pad (hIPFP)-derived mesenchymal stem cells (MSCs) are capable of differentiation into multiple tissue lineages, including cartilage and bone. Chondrogenesis is a crucial part of normal skeletal development but the molecular mechanisms are yet to be completely defined. In this study we sourced hIPFP-derived MSCs utilizing chondrogenic growth factors, transforming growth factor beta-3, and bone morphogenetic protein-6, to form hyaline-like cartilage in micromass cultures and we studied chondrogenic development of 7, 14, and 28 days. The purpos..View full abstract
The authors thank Dr. Rust Turakulov for assistance with microarray data analysis. This project was supported by a grant from the Australian Orthopaedic Association.