Journal article

Gene Expression Variability as a Unifying Element of the Pluripotency Network

Elizabeth A Mason, Jessica C Mar, Andrew L Laslett, Martin F Pera, John Quackenbush, Ernst Wolvetang, Christine A Wells



Heterogeneity is a hallmark of stem cell populations, in part due to the molecular differences between cells undergoing self-renewal and those poised to differentiate. We examined phenotypic and molecular heterogeneity in pluripotent stem cell populations, using public gene expression data sets. A high degree of concordance was observed between global gene expression variability and the reported heterogeneity of different human pluripotent lines. Network analysis demonstrated that low-variability genes were the most highly connected, suggesting that these are the most stable elements of the gene regulatory network and are under the highest regulatory constraints. Known drivers of pluripotenc..

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Awarded by National Heart, Lung, and Blood Institute of the Unites States NIH

Funding Acknowledgements

E.A.M. is supported by an Australian Postgraduate Award scholarship and receives an AIBN student stipend. C. A. W. is supported by a QLD government Smart Futures Fellowship. This work was supported by an ARC special research initiative to Stem Cells Australia (C. A. W., E. W., A. L. L., and M. F. P.) and a grant from the National Heart, Lung, and Blood Institute of the Unites States NIH (1R01HL111759; J.Q.). The authors wish to thank Mr. Othmar Korn and Mr. Rowland Mosbergen for their programming advice and assistance with data processing within the Stemformatics environment.