Metabolite profiling of wheat (Triticum aestivum L.) phloem exudate
Lachlan James Palmer, Daniel Anthony Dias, Berin Boughton, Ute Roessner, Robin David Graham, James Constantine Roy Stangoulis
PLANT METHODS | BMC | Published : 2014
BACKGROUND: Biofortification of staple crops with essential micronutrients relies on the efficient, long distance transport of nutrients to the developing seed. The main route of this transport in common wheat (Triticum aestivum) is via the phloem, but due to the reactive nature of some essential micronutrients (specifically Fe and Zn), they need to form ligands with metabolites for transport within the phloem. Current methods available in collecting phloem exudate allows for small volumes (μL or nL) to be collected which limits the breadth of metabolite analysis. We present a technical advance in the measurement of 79 metabolites in as little as 19.5 nL of phloem exudate. This was achieved ..View full abstract
The authors wish to acknowledge HarvestPlus for helping to fund this work and Metabolomics Australia (School of Botany), funded through Bioplatforms Australia Pty Ltd., a National Collaborative Research Infrastructure Strategy (NCRIS) with co-investment from the Victorian State Government and The University of Melbourne.