Genome-wide DNA methylation profiling identifies a folate-sensitive region of differential methylation upstream of ZFP57-imprinting regulator in humans
Manori Amarasekera, David Martino, Sarah Ashley, Hani Harb, Doerthe Kesper, Deborah Strickland, Richard Saffery, Susan L Prescott
FASEB Journal | FEDERATION AMER SOC EXP BIOL | Published : 2014
Folate intake during pregnancy may affect the regulation of DNA methylation during fetal development. The genomic regions in the offspring that may be sensitive to folate exposure during in utero development have not been characterized. Using genome-scale profiling, we investigated DNA methylation in 2 immune cell types (CD4(+) and antigen-presenting cells) isolated from neonatal cord blood, selected on the basis of in utero folate exposure. High-folate (HF; n=11) and low-folate (LF; n=12) groups were selected from opposite extremes of maternal serum folate levels measured in the last trimester of pregnancy. A comparison of these groups revealed differential methylation at 7 regions across t..View full abstract
R. S. is funded by an Australia National Health and Medical Research Council (NHMRC) Senior Research Fellowship and D. M. by an NHMRC Early Career Fellowship. Murdoch Children's Research Institute is supported by the Victorian government's Operational Infrastructure Scheme. All authors are members of the International Inflammation Network (inFLAME).