Thymosin beta(4) Administration Enhances Fracture Healing in Mice
Rhys D Brady, Brian L Grills, Johannes A Schuijers, Alex R Ward, Brett A Tonkin, Nicole C Walsh, Stuart J McDonald
Journal of Orthopaedic Research® | WILEY-BLACKWELL | Published : 2014
Thymosin β4 (Tβ4 ) is a regenerative peptide that we hypothesized would promote healing of fractured bone. Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline. Calluses from saline- and Tβ4 -treated mice were analyzed for: (1) biomechanical properties and (2) composition using micro-computed tomography (µCT) and histomorphometry. Biomechanical analysis showed that Tβ4 -treated calluses had a 41% increase in peak force to failure (p < 0.01) and were approximately 25% stiffer (p < 0.05) than saline-treated controls. µCT analysis at 21 days post-fracture showed that the fractional volume of new mineralized tissue and new highly mineralize..View full abstract
Grant sponsor: La Trobe University, Faculty of Health Sciences Research Grant.