Diverse Roles for Wnt7a in Ventral Midbrain Neurogenesis and Dopaminergic Axon Morphogenesis
Chathurini V Fernando, Julianna Kele, Christopher R Bye, Jonathan C Niclis, Walaa Alsanie, Brette D Blakely, Jan Stenman, Brad J Turner, Clare L Parish
Stem Cells and Development | MARY ANN LIEBERT, INC | Published : 2014
During development of the central nervous system, trophic, together with genetic, cues dictate the balance between cellular proliferation and differentiation. Subsequent to the birth of new neurons, additional intrinsic and extrinsic signals regulate the connectivity of these cells. While a number of regulators of ventral midbrain (VM) neurogenesis and dopaminergic (DA) axon guidance are known, we identify a number of novel roles for the secreted glycoprotein, Wnt7a, in this context. We demonstrate a temporal and spatial expression of Wnt7a in the VM, indicative of roles in neurogenesis, differentiation, and axonal growth and guidance. In primary VM cultures, and validated in Wnt7a-deficient..View full abstract
This work was supported by grants from the Australian National Health and Medical Research Council (NHMRC) and Victorian-California Stem Cell Alliance grant. B.D.B. was supported by an Australian Postgraduate Award. C.R.B. is an NHMRC Peter Doherty Fellowship. C.L.P. was supported by an NHMRC Career Development Award and subsequent Viertel senior medical research fellowship.