High throughput multichannel fluorescence microscopy with microlens arrays
Antony Orth, Kenneth B Crozier
Optics Express | OPTICAL SOC AMER | Published : 2014
We present a multichannel fluorescence microscopy technique for high throughput imaging applications. A microlens array with over 140,000 elements is used to image centimeter-scale samples at up to 18.1 megapixels per second. Large field-of-view multichannel fluorescent imaging is demonstrated in both sequential and parallel geometries. The extended dynamic range of this approach is also discussed.
Awarded by National Science Foundation
Awarded by Directorate For Engineering
This work was supported partly by the National Science Foundation (grant number ECCS-1201687), and partly by Thermo Fisher Scientific. Fabrication was carried out in the Harvard Center for Nanoscale Systems (CNS), which is supported by the NSF.