Journal article
High throughput multichannel fluorescence microscopy with microlens arrays
A Orth, KB Crozier
Optics Express | Published : 2014
DOI: 10.1364/OE.22.018101
Open access
Abstract
We present a multichannel fluorescence microscopy technique for high throughput imaging applications. A microlens array with over 140,000 elements is used to image centimeter-scale samples at up to 18.1 megapixels per second. Large field-of-view multichannel fluorescent imaging is demonstrated in both sequential and parallel geometries. The extended dynamic range of this approach is also discussed. © 2014 Optical Society of America.
Grants
Awarded by National Science Foundation
Funding Acknowledgements
This work was supported partly by the National Science Foundation (grant number ECCS-1201687), and partly by Thermo Fisher Scientific. Fabrication was carried out in the Harvard Center for Nanoscale Systems (CNS), which is supported by the NSF.