Journal article

Fresnel coherent diffractive imaging tomography of whole cells in capillaries

Mac B Luu, Grant A van Riessen, Brian Abbey, Michael WM Jones, Nicholas W Phillips, Kirstin Elgass, Mark D Junker, David J Vine, Ian McNulty, Guido Cadenazzi, Coralie Millet, Leann Tilley, Keith A Nugent, Andrew G Peele

NEW JOURNAL OF PHYSICS | IOP PUBLISHING LTD | Published : 2014

Abstract

X-ray tomography can be used to study the structure of whole cells in close to their native state. Ptychographic Fresnel coherent diffractive imaging (FCDI) holds particular promise for high-resolution tomographic imaging with quantitative phase sensitivity. To avoid the common missing wedge problem in tomography, cells can be mounted in thin glass capillaries that allow access to the full 180° angular field. However, soft x-rays, which are preferred for cellular imaging, interact strongly with capillaries, sometimes leading to violation of the usual assumptions for coherent diffractive imaging (CDI) and introducing artifacts (i.e., phase wrapping) in the reconstructed images. Here, we descr..

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University of Melbourne Researchers

Grants

Awarded by US Department of Energy, Office of Science


Awarded by US Department of Energy, Office of Basic Energy Sciences


Funding Acknowledgements

The authors acknowledge the support of the Australian Research Council through the Centre of Excellence for Coherent X-Ray Science. The authors acknowledge the use of the Advanced Photon Source at Argonne National Laboratory supported by the US Department of Energy, Office of Science and Office of Basic Energy Sciences, under contract no. DE-AC02-06CH11357. We acknowledge travel funding provided by the International Synchrotron Access Program managed by the Australian Synchrotron and funded by the Australian Government.