Porphyromonas gingivalis Lipopolysaccharide Weakly Activates M1 and M2 Polarized Mouse Macrophages but Induces Inflammatory Cytokines
James A Holden, Troy J Attard, Katrina M Laughton, Ashley Mansell, Neil M O'Brien-Simpson, Eric C Reynolds
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2014
Porphyromonas gingivalis is associated with chronic periodontitis, an inflammatory disease of the tooth's supporting tissues. Macrophages are important in chronic inflammatory conditions, infiltrating tissue and becoming polarized to an M1 or M2 phenotype. As responses to stimuli differ between these phenotypes, we investigated the effect of P. gingivalis lipopolysaccharide (LPS) on M1 and M2 macrophages. M1 and M2 polarized macrophages were produced from murine bone marrow macrophages (BMMϕ) primed with gamma interferon (IFN-γ) or interleukin-4 (IL-4), respectively, and incubated with a low or high dose of P. gingivalis LPS or control TLR2 and TLR4 ligands. In M1-Mϕ, the high dose of P. gin..View full abstract
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Awarded by National Health and Medical Research Council Australia
This work was supported by National Health and Medical Research Council Australia grant APP1029878.