Journal article

Conditional Inactivation of Upstream Binding Factor Reveals Its Epigenetic Functions and the Existence of a Somatic Nucleolar Precursor Body

Nourdine Hamdane, Victor Y Stefanovsky, Michel G Tremblay, Attila Nemeth, Eric Paquet, Frederic Lessard, Elaine Sanij, Ross Hannan, Tom Moss

PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2014

Abstract

Upstream Binding Factor (UBF) is a unique multi-HMGB-box protein first identified as a co-factor in RNA polymerase I (RPI/PolI) transcription. However, its poor DNA sequence selectivity and its ability to generate nucleosome-like nucleoprotein complexes suggest a more generalized role in chromatin structure. We previously showed that extensive depletion of UBF reduced the number of actively transcribed ribosomal RNA (rRNA) genes, but had little effect on rRNA synthesis rates or cell proliferation, leaving open the question of its requirement for RPI transcription. Using gene deletion in mouse, we now show that UBF is essential for embryo development beyond morula. Conditional deletion in cel..

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Grants

Awarded by Canadian Institutes of Health Research (CIHR)


Awarded by Deutsche Forschungsgemeinschaft (DFG)


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

To complete this study the authors received funding from the Canadian Institutes of Health Research (CIHR), (#MOP12205 http://www.cihr-irsc.gc.ca/e/193.html) to TM, the Deutsche Forschungsgemeinschaft (DFG), (#SFB960 http://www.dfg.de/en/) to AN, and the National Health and Medical Research Council of Australia (#1022402, 1038852, #1038852, #1043143 https://www.nhmrc.gov.au) to RH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.