A routine method for cloning, expressing and purifying A beta(1-42) for structural NMR studies
Daniel K Weber, Marc-Antoine Sani, John D Gehman
Amino Acids | SPRINGER WIEN | Published : 2014
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Awarded by ARC
Awarded by LIEF
The authors would like to sincerely thank Dr. Nick Williamson, Paul O'Donnell and Michael Leeming for discussions regarding ESI-MS acquisition and analysis, John Karas for advice on HPLC purification, and Professor Anthony Wedd and Dr. Zhiguang Xiao for allowing access to equipment required for cell-culture work. J. Gehman was partially funded by ARC Future Fellowship FT0991558 for this work. Circular Dichroism and Dynamic Light Scattering instruments were funded by a LIEF grant LE120100186 to G. Bryant (RMIT) and J. Gehman. D. Weber is thankful for an Australian Postgraduate Award PhD scholarship and Dowd Foundation Postgraduate Research Scholarship for Neuroscience.