Journal article
The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like Factor 5 (KLF5)-defendent manner
J Shin, A Carr, GA Corner, L Tögel, M Dávaos-Salas, H Tran, AC Chueh, S Al-Obaidi, F Chionh, N Ahmed, DD Buchanan, JP Young, MS Malo, RA Hodin, D Arango, OM Sieber, LH Augenlicht, AS Dhillon, TK Weber, JM Mariadason
Journal of Biological Chemistry | Published : 2014
Abstract
The histone deacetylase inhibitor (HDACi) sodium butyrate promotes differentiation of colon cancer cells as evidenced by induced expression and enzyme activity of the differentiation marker intestinal alkaline phosphatase (ALPi). Screening of a panel of 33 colon cancer cell lines identified cell lines sensitive (42%) and resistant (58%) to butyrate induction of ALP activity. This differential sensitivity was similarly evident following treatment with the structurally distinct HDACi, MS-275. Resistant cell lines were significantly enriched for those harboring the CpG island methylator phenotype (p = 0.036, Chi square test), and resistant cell lines harbored methylation of the ALPi promoter, p..
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Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
[ "This work was supported in part by the Operational Infrastructure Support Program (Victorian government, Australia), Spanish Ministry for Economy and Competitiveness Grants CP05/00256, TRA2009-0093, SAF2008-00789, PI12/03112, and PI12/01095, the Association for International Cancer Research Grant AICR13-0245, Agencia de Gestio d'Ajuts Universitaris i de Recerca SGR 157 (to D. A.), and Australian Research Council Future Fellowship FT0992234 and National Health and Medical Research Council Senior Research Fellowship 1046092 (to J. M. M.).", "Supported by a fellowship from CONACyT Mexico (186310)." ]