Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser
Christopher Kupitz, Shibom Basu, Ingo Grotjohann, Raimund Fromme, Nadia A Zatsepin, Kimberly N Rendek, Mark S Hunter, Robert L Shoeman, Thomas A White, Dingjie Wang, Daniel James, Jay-How Yang, Danielle E Cobb, Brenda Reeder, Raymond G Sierra, Haiguang Liu, Anton Barty, Andrew L Aquila, Daniel Deponte, Richard A Kirian Show all
Nature | NATURE PUBLISHING GROUP | Published : 2014
Awarded by Center for Bio-Inspired Solar Fuel Production, an Energy Frontier Research Center - DOE, Office of Basic Energy Sciences
Awarded by National Institutes of Health
Awarded by US National Science Foundation
Awarded by DFG Clusters of Excellence 'Inflammation at Interfaces'
Awarded by US DOE through Lawrence Livermore National Laboratory
Awarded by UCOP Lab Fee Program
Awarded by LLNL LDRD program
Awarded by U.S. Department of Energy (DOE), Office of Basic Energy Sciences
Awarded by National Science Foundation Graduate Research Fellowship
Awarded by National Science Foundation through the BioFEL Science Technology Center
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
Experiments were carried out at the Linac Coherent Light Source (LCLS), a national user facility operated by Stanford University on behalf of the US Department of Energy (DOE), Office of Basic Energy Sciences (OBES). This work was supported by the following agencies: the Center for Bio-Inspired Solar Fuel Production, an Energy Frontier Research Center funded by the DOE, Office of Basic Energy Sciences (award DE-SC0001016), the National Institutes of Health (award 1R01GM095583), the US National Science Foundation (award MCB-1021557 and MCB-1120997), the DFG Clusters of Excellence 'Inflammation at Interfaces' (EXC 306) and the 'Center for Ultrafast Imaging'; the Deutsche Forschungsgemeinschaft (DFG); the Max Planck Society, the Atomic, Molecular and Optical Sciences Program; Chemical Sciences Geosciences and Biosciences Division, DOE OBES (M.J.B.) and the SLAC LDRD program (M.J.B., H.L.); the US DOE through Lawrence Livermore National Laboratory under the contract DE-AC52-07NA27344 and supported by the UCOP Lab Fee Program (award no. 118036) and the LLNL LDRD program (12-ERD-031); the Hamburg Ministry of Science and Research and Joachim Herz Stiftung as part of the Hamburg Initiative for Excellence in Research. The research at Purdue University was supported by the U.S. Department of Energy (DOE), Office of Basic Energy Sciences DE-FG02-12ER16340 (Y.P.) and the National Science Foundation Graduate Research Fellowship under Grant 0833366 (K.M.D.). We also want to thank the National Science Foundation for providing funding for the publication of this work through the BioFEL Science Technology Center (award 1231306). We thank H. Isobe, M. Shoji, S. Yamanaka, Y. Umena, K. Kawakami, N. Kamiya, J. R. Shen and K. Yamaguchi for permission to show a section of Fig. 6 of their publication ref. 4 in Fig. 3d of this publication. We thank R. Neutze and his team for support and discussions during joint beamtime for the PSII project and his projects on time-resolved wide-angle scattering studies. We thank A. T. Brunger for discussions concerning data analysis. We thank T. Terwilliger for support with parameter setting of phenix.autobuild program for the SA-omit maps. We also wish to thank R. Burnap for discussions concerning interpretation of results of ligand mutagenesis. We thank J. D. Zook for his contributions concerning plastoquinone quantification. We thank M. Zhu for helping to create high resolution figures for this publication. We thank Raytheon for support of our studies by providing night-vision devices.