Journal article
Bioinformatic exploration of RIO protein kinases of parasitic and free-living nematodes
B Breugelmans, AR Jex, PK Korhonen, S Mangiola, ND Young, PW Sternberg, PR Boag, A Hofmann, RB Gasser
International Journal for Parasitology | ELSEVIER SCI LTD | Published : 2014
Abstract
Despite right open reading frame kinases (RIOKs) being essential for life, their functions, substrates and cellular pathways remain enigmatic. In the present study, gene structures were characterised for 26 RIOKs from draft genomes of parasitic and free-living nematodes. RNA-seq transcription profiles of riok genes were investigated for selected parasitic nematodes and showed that these kinases are transcribed in developmental stages that infect their mammalian host. Three-dimensional structural models of Caenorhabditis elegans RIOKs were predicted, and elucidated functional domains and conserved regions in nematode homologs. These findings provide prospects for functional studies of riok ge..
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Awarded by Australian Research Council
Funding Acknowledgements
This project was funded by the National Health and Medical Research Council (NHMRC) of Australia, and the Australian Research Council (ARC). This project was also supported by a Victorian Life Sciences Computation Initiative (VLSCI), Australia, grant number VR0007 on its Peak Computing Facility at the University of Melbourne, Australia, an initiative of the Victorian Government. Other support from the Australian Academy of Science, the Australian-American Fulbright Commission, Alexander von Humboldt Foundation, Germany, Melbourne Water Corporation, Australia (R.B.G.) is gratefully acknowledged, as is funding from the Howard Hughes Medical Institute (HHMI), USA, and National Institutes of Health (NIH), USA (P.W.S.). N.D.Y is an NHMRC Early Career Research Fellow (ECRF). Thanks to Brendan Ansell for discussions and advice on the use of I-TASSER. We also acknowledge the contributions of all staff at WormBase (www.wormbase.org). Data for S. ratti (GenBank assembly ID: GCA_000208845.1) and B. malayi (RNA-seq, PRJEB2709) were kindly provided by the Wellcome Trust Sanger Institute, UK (http://www.ebi.ac.uk/ena/data/view/PRJEB2709). We acknowledge the use of the program I-TASSER 2013 The Regents of the University of Michigan, USA.