Journal article

Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

Kay Richards, Fernando Calamante, Jacques-Donald Tournier, Nyoman D Kurniawan, Farnoosh Sadeghian, Alexander R Retchford, Gabriel Davis Jones, Christopher A Reid, David C Reutens, Roger Ordidge, Alan Connelly, Steven Petrou



In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20 μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant wit..

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Awarded by Australian Research Council

Awarded by National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

This work was supported by the Victorian Government through the Operational Infrastructure Scheme. We thank the Queensland NMR Network (QNN) and the National Imaging Facility (NIF) for instrument access and technical support. This work was funded by the Australian Research Council (ARC DP120104112; FT110100726; CE140100007), the National Health and Medical Research Council (NHMRC Program Grant 628952) and fellowship (1005050).