Slit2 is decreased after spontaneous labour in myometrium and regulates pro-labour mediators
Ratana Lim, Stella Liong, Gillian Barker, Martha Lappas
JOURNAL OF REPRODUCTIVE IMMUNOLOGY | ELSEVIER IRELAND LTD | Published : 2014
Preterm birth, a global healthcare problem, is commonly associated with inflammation. As Slit2 plays an emerging role in inflammation, the purpose of this study was to determine the effect of Slit2 on labour mediators in human gestational tissues. Slit2 mRNA and protein expression were assessed using qRT-PCR and immunohistochemistry in foetal membranes and myometrium obtained before and after labour. Slit2 silencing was achieved using siRNA in primary myometrial cells. Pro-inflammatory and pro-labour mediators were evaluated by qRT-PCR, ELISA and gelatin zymography. Slit2 mRNA and protein expression were found to be significantly lower in myometrium after labour onset. There was no effect of..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC)
Associate Professor Martha Lappas is supported by a Career Development Fellowship from the National Health and Medical Research Council (NHMRC; grant no. 1047025). Dr. Ratana Lim was, in part, supported by an early career researcher grant from the University of Melbourne. Additional funding was provided from the Mercy Research Foundation. Funding for the iMark (TM) microplate spectrophotometer and the ChemiDoc system and was provided by the Medical Research Foundation for Women and Babies.