Journal article
A Cytoprotective and Degradable Metal-Polyphenol Nanoshell for Single-Cell Encapsulation
Ji Hun Park, Kyunghwan Kim, Juno Lee, Ji Yu Choi, Daewha Hong, Sung Ho Yang, Frank Caruso, Younghoon Lee, Insung S Choi
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | WILEY-V C H VERLAG GMBH | Published : 2014
Abstract
Single-cell encapsulation promises the cytoprotection of the encased cells against lethal stressors, reminiscent of the sporulation process in nature. However, the development of a cytocompatible method for chemically mimicking the germination process (i.e., shell degradation on-demand) has been elusive, despite the shell degradation being pivotal for the practical use of functional cells as well as for single cell-based biology. We report that an artificial shell, composed of tannic acid (TA) and Fe(III) , on individual Saccharomyces cerevisiae controllably degrades on-demand, while protecting the yeast from multiple external aggressors, including UV-C irradiation, lytic enzymes, and silver..
View full abstractGrants
Awarded by Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning (MSIP)
Awarded by Intelligent Synthetic Biology Center of Global Frontier Project - MSIP
Awarded by Australian Research Council under the Australian Laureate Fellowship Scheme
Funding Acknowledgements
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP) (2012R1A3A2026403 to I. S. C.; 2011-0020322 to Y.L.), the Intelligent Synthetic Biology Center of Global Frontier Project funded by MSIP (2012M3A6A8055678 to Y.L.), and the Australian Research Council under the Australian Laureate Fellowship Scheme (FL120100030 to F. C.). M. S. Jung at the KBSI (Chuncheon) is thanked for technical assistance with the preparation of microtome-sliced samples.