Culture of Viable Mammalian Embryos In Vitro

Abstract

The field of modern embryo culture spans several decades. However, in the last decade of the 20th century a resurgence of interest in embryo physiology and metabolism led to highly significant improvements in embryo culture conditions. It also became apparent that for successful development of viable mammalian embryos in vitro, one needs to understand that culture conditions go far beyond the composition of the media used. Rather, it is imperative to appreciate the many variables of the culture system, each of which is capable of impacting the development and viability of the preimplantation embryo. By taking a more holistic approach to embryo culture, it is not only possible to attain high ..