Journal article

Modified Vaccinia Virus Ankara Encoding Influenza Virus Hemagglutinin Induces Heterosubtypic Immunity in Macaques

Nicholas W Florek, Jason T Weinfurter, Sinthujan Jegaskanda, Joseph N Brewoo, Tim D Powell, Ginger R Young, Subash C Das, Masato Hatta, Karl W Broman, Olav Hungnes, Susanne G Dudman, Yoshihiro Kawaoka, Stephen J Kent, Dan T Stinchcomb, Jorge E Osorio, Thomas C Friedrich

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2014

Abstract

UNLABELLED: Current influenza virus vaccines primarily aim to induce neutralizing antibodies (NAbs). Modified vaccinia virus Ankara (MVA) is a safe and well-characterized vector for inducing both antibody and cellular immunity. We evaluated the immunogenicity and protective efficacy of MVA encoding influenza virus hemagglutinin (HA) and/or nucleoprotein (NP) in cynomolgus macaques. Animals were given 2 doses of MVA-based vaccines 4 weeks apart and were challenged with a 2009 pandemic H1N1 isolate (H1N1pdm) 8 weeks after the last vaccination. MVA-based vaccines encoding HA induced potent serum antibody responses against homologous H1 or H5 HAs but did not stimulate strong T cell responses pri..

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University of Melbourne Researchers

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Awarded by NIH


Awarded by NIH National Research Service Award


Awarded by NIH Research Facilities Improvement Program


Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Awarded by OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH


Funding Acknowledgements

This work was supported by a supplement to the WNPRC base grant (NIH P51 RR000167/OD011106), awarded to T.C.F., and by a donation to the University of Wisconsin School of Veterinary Medicine from Inviragen, Inc. N.W.F. was supported by NIH National Research Service Award T32 GM07215. This research was conducted in part at a facility constructed with support from NIH Research Facilities Improvement Program grants RR015459 and RR020141.