Journal article

VP7 of Rhesus monkey rotavirus RRV contributes to diabetes acceleration in association with an elevated anti-rotavirus antibody response

Jessica A Pane, Vi T Dang, Gavan Holloway, Nicole L Webster, Barbara S Coulson



T cell-receptor transgenic NOD8.3 mice provide a model for spontaneous type 1 diabetes development. Infection of 5 week-old NOD8.3 mice with Rhesus monkey rotavirus (RRV) accelerates the onset of their diabetes. This acceleration requires virus replication and relates to the presence and level of serum anti-rotavirus antibodies, but the role of individual RRV genes is unknown. Here we assessed the importance for diabetes acceleration of the RRV genes encoding VP4 and VP7, by infecting NOD8.3 mice with parental and reassortant rotaviruses. Diabetes was accelerated by reassortant rotaviruses containing RRV VP7 on a UK rotavirus genetic background, but not by parental UK or a UK reassortant con..

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Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

We are grateful to Harry Greenberg and Yasutaka Hoshino for provision of rotavirus reassortants 12/1 and 28/1, and 9-8-1, respectively, and Kate Graham for assistance with the CRW-8 studies. Pere Santamaria graciously provided the NOD8.3 mouse line. The mouse husbandry of David Taylor, Rhiannon Hall, Rebecca Bowyer and Katarina Parr is much appreciated. This work was supported by Project Grant 1044868 and a Senior Research Fellowship (628319) to B. S. C. from the National Health and Medical Research Council of Australia.