In vivo assessment of grafted cortical neural progenitor cells and host response to functionalized self-assembling peptide hydrogels and the implications for tissue repair
AL Rodriguez, TY Wang, KF Bruggeman, CC Horgan, R Li, RJ Williams, CL Parish, DR Nisbet
Journal of Materials Chemistry B | ROYAL SOC CHEMISTRY | Published : 2014
Tissue specific scaffolds formed from minimalist N-fluorenylmethyloxycarbonyl self-assembling peptides (Fmoc-SAPs) have emerged as promising biomaterials due to their ease of synthesis and capacity to self-assemble via simple, non-covalent interactions into complex nanofibrous hydrogels. However, concerns remain over their biocompatibility and cytotoxicity for in vivo applications. Here, we demonstrate that these Fmoc-SAPs are biocompatible in vivo and well suited as a delivery vehicle for cell transplantation. In order to determine the effect of tissue specific parameters, we designed three Fmoc-SAPs containing varying bioactive peptide sequences derived from extracellular matrix proteins, ..View full abstract
The acknowledgements come at the end of an article after the We would like to thank Dr Navdeep Kaur and Joanne Lee (Centre for Advanced Microscopy, ANU) for their help and guidance in preparing negative stains for TEM imaging and Francesca Maclean and Shayanti Mukerhjee for thorough proof reading of the manuscript. This research was supported by funding from the National Health and Medical Research Council (NHMRC), Australia and the Australian Research Council. ALR was supported by an Australian Postgraduate Award; CLP was supported by an NHMRC Career Development Award, and subsequently Senior Medical Research Fellowship provided by the Viertel charitable Foundation, Australia; DRN was supported by an Australian Research Council Australian Postdoctoral Fellowship, and subsequently by a NHMRC Career Development Fellowship; and RJW is funded via an Alfred Deakin Research Fellowship. The Florey Institute of Neuroscience and Mental Health acknowledges the support from the Victorian Government's Operational Infrastructure Support Grant.