Journal article
Proteomics elucidates key molecules involved in exsheathment in vitro in Oesophagostomum dentatum
M Ondrovics, K Silbermayr, M Mitreva, ND Young, RB Gasser, A Joachim
International Journal for Parasitology | Published : 2014
Abstract
We explored molecules involved in in vitro exsheathment of Oesophagostomum dentatum L3s using a proteomic-transcriptomic-bioinformatic approach. Analysis of L3s before, during and after exsheathment identified 11 proteins that were over-expressed exclusively during exsheathment. These proteins (including key enzymes, heat shock, structural and nematode-specific proteins) were inferred to be involved in development, metabolism, structure, motility and/or host-parasite interactions. Some of these molecules represented homologues linked to entry into and exit from the dauer stage in the free-living nematode Caenorhabditis elegans. The approach established here provides a basis for investigation..
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Awarded by National Institutes of Health
Funding Acknowledgements
MO is recipient of a DOC-fFORTE-fellowship of the Austrian Academy of Sciences. The O. dentatum genome and transcriptome data are generated as part of the Strongylida genome sequencing project funded by National Institutes of Health (NIH), USA (grant number AI081803). RBG's research is funded mainly through the Australian Research Council (ARC), the National Health and Medical Research Council of Australia (NHMRC), Melbourne Water Corporation, Australia and Yourgene Bioscience, Taiwan, and supported by a Victoria Life Sciences Computation Initiative (VLSCI), Australia (grant number VR0007) on its Peak Computing Facility at The University of Melbourne, Australia, an initiative of the Victorian Government. NDY is an NHMRC Early Career Research Fellow (ECRF). We thank Ebrahim Razzazi-Fazeli, Katharina Nobauer and Karin Hummel (VetCore, University of Veterinary Medicine Vienna, Austria) for their technical support in mass spectrometric analysis. Ingrid Miller (Department for Biomedical Sciences, University of Veterinary Medicine Vienna, Austria) is gratefully acknowledged for assistance with the scanning of 2D-DIGE gels. We also thank Hanna Lucia Worliczek (Institute of Parasitology, University of Veterinary Medicine Vienna) for her technical support with microscopic imaging.