Thrombospondin 1 promotes an aggressive phenotype through epithelial-to-mesenchymal transition in human melanoma
Aparna Jayachandran, Matthew Anaka, Prashanth Prithviraj, Christopher Hudson, Sonja J McKeown, Pu-Han Lo, Laura J Vella, Colin R Goding, Jonathan Cebon, Andreas Behren
ONCOTARGET | IMPACT JOURNALS LLC | Published : 2014
Epithelial-to-mesenchymal transition (EMT), in which epithelial cells loose their polarity and become motile mesenchymal cells, is a determinant of melanoma metastasis. We compared gene expression signatures of mesenchymal-like melanoma cells with those of epithelial-like melanoma cells, and identified Thrombospondin 1 (THBS1) as highly up-regulated in the mesenchymal phenotype. This study investigated whether THBS1, a major physiological activator of transforming growth factor (TGF)-beta, is involved in melanoma EMT-like process. We sought to examine expression patterns in distinct melanoma phenotypes including invasive, de-differentiated, label-retaining and drug resistant populations that..View full abstract
We would like to acknowledge the Melanoma Research Alliance (MRA) and the Victorian State Government Operational Infrastructure Support Program for partial funding of this project. AB and AJ were supported by Cure Cancer Australia Foundation Fellowships. JC was supported by Australian National Health and Medical Research Council Practitioner Fellowship. The establishment of an expression profile across 58 melanoma cell lines was funded by a grant from the Austin Hospital Research Foundation to AB.