Journal article

Relaxin-3 receptor (Rxfp3) gene knockout mice display reduced running wheel activity: Implications for role of relaxin-3/RXFP3 signalling in sustained arousal

Ihaia T Hosken, Steven W Sutton, Craig M Smith, Andrew L Gundlach



Anatomical and pharmacological evidence suggests the neuropeptide, relaxin-3, is the preferred endogenous ligand for the relaxin family peptide-3 receptor (RXFP3) and suggests a number of putative stress- and arousal-related roles for RXFP3 signalling. However, in vitro and in vivo evidence demonstrates exogenous relaxin-3 can activate other relaxin peptide family receptors, and the role of relaxin-3/RXFP3 signalling in specific brain circuits and associated behaviours in mice is not well described. In this study, we characterised the behaviour of cohorts of male and female Rxfp3 gene knockout (KO) mice (C57/B6J(RXFP3TM1/DGen)), relative to wild-type (WT) littermates to determine if this rec..

View full abstract

University of Melbourne Researchers


Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This research was supported by National Health and Medical Research Council of Australia project grants 509246, 1005985 and 1024885 (A.L.G.) and postgraduate scholarship 1017408 (I.T.H.), grants from the Pratt and Besen Family Foundations (A.L.G.), a Brain & Behavior Research Foundation (USA) NARSAD Independent Investigator Award (A.L.G.), and the Victorian Government Operational Infrastructure Support Programme. The authors thank Dr Timothy Lovenberg (Neuroscience Drug Discovery, Janssen Pharmaceutical Companies of Johnson & Johnson, San Diego, USA) for commissioning the production of the Rxfp3 KO strain and providing The Florey Institute of Neuroscience and Mental Health with an original breeding colony; and Simon Miller and Brett Purcell (The Florey Institute of Neuroscience and Mental Health) for assistance with automated running wheel production and maintenance, and assistance with behavioural equipment and analysis, respectively.