Journal article
Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp: And Salmonella enterica in sheep
R Yang, C Jacobson, G Gardner, I Carmichael, AJD Campbell, U Ryan
Veterinary Journal | Published : 2014
Abstract
Faecal excretion of Campylobacter spp. and Salmonella enterica in sheep in Australia was determined using a quantitative multiplex PCR (qPCR) targeting the Campylobacter spp. purine biosynthesis gene (PurA) and the S. enterica outer membrane protein (ompF). The mutiplex qPCR was specific and Campylobacter spp. and S. enterica were each detected with a sensitivity of 5 organisms/μL faecal DNA extract. This multiplex qPCR was used to determine the prevalence and concentration of Campylobacter spp. and S. enterica in 3412 faecal samples collected from 1189 lambs on eight farms across South Australia (n = 2 farms), New South Wales (n = 1), Victoria (n = 2) and Western Australia (n = 3) at three ..
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Awarded by Australian Wool Innovation
Funding Acknowledgements
This study was funded by Meat and Livestock Australia (MLA), Australian Wool Innovation Limited (AWI) and the Australian Government (project number AHE.0027). We thank the participating farmers for their support and for providing access to sheep for sample collection. We thank Justin Hoad for providing faecal samples from NSW. Samples from the WA farms were collected and DNA extracted by Joshua Sweeny. Thanks also go to Josephine Ng-Hublin for DNA extraction of faecal samples collected from the eastern states.