Journal article

Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination

YL Xing, PT Röth, JAS Stratton, BHA Chuang, J Danne, SL Ellis, SW Ng, TJ Kilpatrick, TD Merson

Journal of Neuroscience | Published : 2014

Abstract

Parenchymal oligodendrocyte progenitor cells (pOPCs) are considered the principal cell type responsible for oligodendrogenesis and remyelinaton in demyelinating diseases. Recent studies have demonstrated that neural precursor cells (NPCs) from the adult subven-tricular zone (SVZ) can also generate new oligodendrocytes after demyelination. However, the relative contribution of NPCs versus pOPCs to remyelination is unknown. We used in vivo genetic fate mapping to assess the behavior of each progenitor type within the corpus callosi (CCs) of mice subjected to cuprizone-induced demyelination. Nestin-CreERT2 and Pdgfra-CreERT2 transgenic mice were crossed with fluorescent Cre reporter strains to ..

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Grants

Funding Acknowledgements

This work was supported by a Strategic Research Australian Postgraduate Award (Stem Cells Australia; Y.L.X.), a National Health and Medical Research Council of Australia/Multiple Sclerosis Research Australia Betty Cuthbert Fellowship (T.D.M.), and a Neuroscience Institute Fellowship (T.D.M.); and by Stem Cells Australia-the Australian Research Council Special Research Initiative in Stem Cell Science (T.D.M. and T.J.K.). Charity Works for MS and the Victorian Operational Infrastructure Support Program provided additional support. We thank Professor William Richardson (University College London, London, UK), Professor Ryoichiro Kageyama (University of Kyoto, Kyoto, Japan), and Professor Frank Costantini (Columbia University Medical Center, New York, NY) for generously providing the Pdgfra-CreER<SUP>T2</SUP>, Nestin-CreER<SUP>T2</SUP> line 5-1, and Rosa26-eYFP transgenic mouse lines, respectively.