Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.
Niliksha Gunewardene, Nicole Van Bergen, Duncan Crombie, Karina Needham, Mirella Dottori, Bryony A Nayagam
BioResearch Open Access | Published : 2014
Emerging therapies for sensorineural hearing loss include replacing damaged auditory neurons (ANs) using stem cells. Ultimately, it is important that these replacement cells can be patient-matched to avoid immunorejection. As human induced pluripotent stem cells (hiPSCs) can be obtained directly from the patient, they offer an opportunity to generate patient-matched neurons for transplantation. Here, we used an established neural induction protocol to differentiate two hiPSC lines (iPS1 and iPS2) and one human embryonic stem cell line (hESC; H9) toward a neurosensory lineage in vitro. Immunocytochemistry and qRT-PCR were used to analyze the expression of key markers involved in AN developmen..View full abstract