Some properties of feline panleukopenia virus

Abstract

Feline panleukopenia virus was isolated from a peracute, fatal disease in a 3 month old Burmese kitten by inoculation of a 1% spleen suspension onto freshly seeded cultures of a feline embryo (FEmb) cell line. The virus was assayed by the detection of intranuclear inclusion bodies in stained coverslips of FEmb cells. The virus was not inactivated by ether, pH 3 or 56° C for 2 hours. The titer of the virus was reduced from 5.01 x 106 to 1.58 x 104 50% tissue culture infective doses when grown in the presence of 20 μg/ml 2 bromo 5 deoxyuridine. The identity of the isolated virus as a strain of panleukopenia was determined by showing that it was antigenically related to a standard vaccine strai..