The Porphyromonas gingivalis Ferric Uptake Regulator Orthologue Binds Hemin and Regulates Hemin-Responsive Biofilm Development
Catherine A Butler, Stuart G Dashper, Lianyi Zhang, Christine A Seers, Helen L Mitchell, Deanne V Catmull, Michelle D Glew, Jacqueline E Heath, Yan Tan, Hasnah SG Khan, Eric C Reynolds
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014
Porphyromonas gingivalis is a Gram-negative pathogen associated with the biofilm-mediated disease chronic periodontitis. P. gingivalis biofilm formation is dependent on environmental heme for which P. gingivalis has an obligate requirement as it is unable to synthesize protoporphyrin IX de novo, hence P. gingivalis transports iron and heme liberated from the human host. Homeostasis of a variety of transition metal ions is often mediated in Gram-negative bacteria at the transcriptional level by members of the Ferric Uptake Regulator (Fur) superfamily. P. gingivalis has a single predicted Fur superfamily orthologue which we have designated Har (heme associated regulator). Recombinant Har forme..View full abstract
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Awarded by Australian National Health and Medical Research Council
Awarded by Australian Dental Research Foundation
Financial assistance was received from The Australian National Health and Medical Research Council (https://www.nhmc.gov.au/) project grant 1008055 and Australian Dental Research Foundation (http://www.ada.org.au/about/adrfgrants.aspx) grant 22/2002. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.