Different Neural Crest Populations Exhibit Diverse Proliferative Behaviors
David G Gonsalvez, Mathew Li-Yuen-Fong, Kylie N Cane, Lincon A Stamp, Heather M Young, Colin R Anderson
DEVELOPMENTAL NEUROBIOLOGY | WILEY-BLACKWELL | Published : 2015
The rate of proliferation of cells depends on the proportion of cycling cells and the frequency of cell division. Here, we describe in detail methods for quantifying the proliferative behavior of specific cell types in situ, and use the method to examine cell cycle dynamics in two neural crest derivatives--dorsal root ganglia (DRG) using frozen sections, and the enteric nervous system (ENS) using wholemount preparations. In DRG, our data reveal a significant increase in cell cycle length and a decrease in the number of cycling Sox10+ progenitor cells at E12.5-E13.5, which coincides with the commencement of glial cell generation. In the ENS, the vast majority of Sox10+ cells remain proliferat..View full abstract
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Awarded by NHMRC Senior Research Fellowship
Contract grant sponsor: NHMRC Project; contract grant number: 1063822 (C.R.A. and H.M.Y.) and 1047953 (H.M.Y.).Contract grant sponsor: NHMRC Senior Research Fellowship; contract grant number: 1002506 (H.M.Y.).