Identification of binding sites for C-terminal pro-gastrin-releasing peptide (GRP)-derived peptides in renal cell carcinoma: a potential target for future therapy

Joseph Ischia; Oneel Patel; Kapil Sethi; Marianne S Nordlund; Damien Bolton; Arthur Shulkes; Graham S Baldwin

Journal article

Identification of binding sites for C-terminal pro-gastrin-releasing peptide (GRP)-derived peptides in renal cell carcinoma: a potential target for future therapy

Joseph Ischia, Oneel Patel, Kapil Sethi, Marianne S Nordlund, Damien Bolton, Arthur Shulkes, Graham S Baldwin

BJU International | WILEY-BLACKWELL | Published : 2015

DOI: 10.1111/bju.12886

Abstract

OBJECTIVE: To determine the expression and biology of the neuroendocrine growth factor gastrin-releasing peptide (GRP) and other proGRP-derived peptides in renal cancer. MATERIALS AND METHODS: Receptor binding studies, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, were used to quantitate the presence of proGRP-derived peptide receptors and their ligands in renal cancer cell lines and human renal cancers. Biological activity of proGRP peptides was confirmed with proliferation, migration, and extracellular-signal-regulated kinases 1 and 2 (ERK1/2) activation assays in vitro. In vivo, ACHN renal cancer xenografts were treated with proGRP-derived peptides to assess tumour size ..

View full abstract

University of Melbourne Researchers

Graham Baldwin Author Surgery - Austin Health

Oneel Patel Author Surgery - Austin Health

Arthur Shulkes Author Surgery - Austin Health

Joseph Ischia Author Surgery - Austin Health

Damien Bolton Author Surgery - Austin Health

Related Projects (5)

Biology of GRP precursors

Colorectal carcinoma (cancer of the large bowel) is the second most common cause of cancer death. Colorectal carcinomas in common with other..

RESEARCH FELLOWSHIP - PROF ARTHUR SHULKES

I am a physiologist with expertise in endocrinology determining the roles of particular regulatory peptides in the normal functions of the g..

Senior Principal Research Fellowship Renewal

I am a physiologist with expertise in endocrinology determining the roles of particular regulatory peptides in the normal functions of the g..

Role of the Growth Factor ProGRP in the Development of Gastrointestinal Cancers

Our objective is to determine the roles of a growth factor termed bombesin. This peptide, known as GRP in the human, is a growth factor in c..

RESEARCH FELLOWSHIP 2007 - BALDWIN

I am a biochemist-cell biologist, working on the growth factors of the gastrointestinal tract. My discovery that the activity of non-amidate..

Grants

Awarded by Austin Health Medical Research Foundation

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

We thank Mildred Yim and Mike Chang for skilled assistance with radioimmunoassay and ELISA, respectively. This work was supported by grants from the Austin Health Medical Research Foundation and by grants 454408 and 628390 (to G.B and A.S.), 454322 (to G.B.) and 350235 (to A.S.) from the National Health and Medical Research Council of Australia. JI was supported by research scholarships from the NHMRC and the Royal Australian College of Surgeons.