Journal article
Impact of conditional deletion of the pro-apoptotic BCL-2 family member BIM in mice
MJ Herold, R Stuchbery, D Mérino, T Willson, A Strasser, D Hildeman, P Bouillet
Cell Death and Disease | Published : 2014
Abstract
The pro-apoptotic BH3-only BCL-2 family member BIM is a critical determinant of hematopoietic cell development and homeostasis. It has been argued that the striking hematopoietic abnormalities of BIM-deficient mice (accumulation of lymphocytes and granulocytes) may be the result of the loss of the protein throughout the whole animal rather than a consequence intrinsic to the loss of BIM in hematopoietic cells. To address this issue and allow the deletion of BIM in specific cell types in future studies, we have developed a mouse strain with a conditional Bim allele as well as a new Cre transgenic strain, Vav-CreER, in which the tamoxifen-inducible CreER recombinase (fusion protein) is predomi..
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Awarded by National Institute of Arthritis and Musculoskeletal and Skin Diseases
Funding Acknowledgements
We thank M Robati for technical assistance, Dr LA O'Reilly for sharing reagents and expertise, G Siciliano and S O'Connor for mouse care, J Corbin for automated blood analysis, B Helbert and C Young for genotyping. This work was supported by the Australian NHMRC (Program Grant 461221, Independent Research Institutes Infrastructure Support Scheme Grant 361646, Research Fellowship 1042629 and Project Grant 1049720 (MJH)), the Leukemia and Lymphoma Society (Specialised Center of Research Grant 7015), and infrastructure support from the NHMRC (IRISS) and the Victorian State Government (OIS).