Journal article

T lymphocytes from granulocyte colony-stimulating factor(-/-) mice produce large quantities of interferon-gamma in a chronic infection model

SI Mannering, Y Zhan, B Gilbertson, GJ Lieschke, C Cheers

IMMUNOLOGY | BLACKWELL SCIENCE LTD | Published : 2000

Abstract

Little is known about the role of granulocyte colony-stimulating factor (G-CSF) in the response to chronic bacterial infections. To address this we infected G-CSF knock out (G-CSF-/-) mice with Mycobacterium avium. Infection was not exacerbated in G-CSF-/- mice despite a deficiency in the total bone marrow cells, colony-forming haemopoietic cells, granulocytes and monocyte precursors in the bone marrow. Peritoneal cells from G-CSF-/- produced less nitric oxide (NO) upon culture in vitro with antigen than did wild-type (WT) cells. Unexpectedly, T cells from infected G-CSF-/- mice were able to produce significantly more interferon-gamma (IFN-gamma) than the wild type (WT) controls. T cells fro..

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University of Melbourne Researchers