Development of second generation peptides modulating cellular adiponectin receptor responses
Laszlo Otvos, Daniel Knappe, Ralf Hoffmann, Ilona Kovalszky, Julia Olah, Tim D Hewitson, Roma Stawikowska, Maciej Stawikowski, Predrag Cudic, Feng Lin, John D Wade, Eva Surmacz, Sandor Lovas
Frontiers in Chemistry | FRONTIERS MEDIA SA | Published : 2014
The adipose tissue participates in the regulation of energy homeostasis as an important endocrine organ that secretes a number of biologically active adipokines, including adiponectin. Recently we developed and characterized a first-in-class peptide-based adiponectin receptor agonist by using in vitro and in vivo models of glioblastoma and breast cancer (BC). In the current study, we further explored the effects of peptide ADP355 in additional cellular models and found that ADP355 inhibited chronic myeloid leukemia (CML) cell proliferation and renal myofibroblast differentiation with mid-nanomolar IC50 values. According to molecular modeling calculations, ADP355 was remarkably flexible in th..View full abstract
Awarded by National Institutes of Health
Awarded by NIH
This work was funded partially from a subcontract (to Laszlo Otvos) of National Institutes of Health grant AR064925 (Principal Investigator Dr. John Varga, Northwestern University). John D. Wade is an NHMRC (Australia) Principal Research Fellow. Research at the Florey Institute of Neuroscience and Mental Health is supported by the Victorian Government Operational Infrastructure Support Program. Sandor Lovas was supported by NIH grants RR016469 and GM103427. We are grateful to Dr. Yuguang Mu for providing the dPCA analysis code.