Journal article

SIGNALS CONTROLLING ALTERNATIVE SPLICING OF MAJOR HISTOCOMPATIBILITY COMPLEX H-2 CLASS-I PRE-MESSENGER RNA

DE HANDY, J MCCLUSKEY, AM LEW, JE COLIGAN, DH MARGULIES

IMMUNOGENETICS | SPRINGER VERLAG | Published : 1988

Abstract

The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein: the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 bp from the splice junction and is contained in the sequence AGTGATGG. D-region genes, which use only the downstream splice site, have the sequence AGTGGTGG. We have used in vitro mutagenesis..

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