Signals controlling alternative splicing of major histocompatibility complex H-2 class I pre-mRNA

Abstract

The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein: the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 by from the splice junction and is contained in the sequence AGTG ATGG. D-region genes, which use only the downstream splice site, have the sequence AGTG GTGG. We have used in vitro mutagenes..