Analysis of hybrid H-2D and L antigens with reciprocally mismatched aminoterminal domains: Functional T cell recognition requires preservation of fine structural determinants

Abstract

Studies of immune recognition of hybrid class I antigens expressed on transfected cells have revealed an apparent general requirement that the N(α1) and C1(α2) domains be derived from the same gene in order to preserve recognition by virus-specific H-2-restricted and allospecific T cells. One exception has been the hybrid DL antigen in which the N domain of H-2L(d) has been replaced by that of H-2D(d). Cells bearing this molecule serve as targets for some virus and allospecific CTL. Because cells expressing the reciprocal hybrid LD (N domain of H-2D(d) replaced by that of H-2L(d)) antigen have not been available, it has not been possible to evaluate whether this exception stemmed from the re..