Serologic and T cell recognition of truncated transplantation antigens encoded by in vitro deleted class I major histocompatibility genes

Abstract

Gene constructions encoding a leader sequence and the C2, transmembrane, and intracytoplasmic exons of both H-2L(d) (pL(d)-C2) and H-2D(d) (pD(d)-C2) were expressed as cell surface proteins when stably introduced into mouse L cells. Distinct proteins of 17.5 and 16 kd precipitated from pD(d)-C2 transformants reacted with a C2 domain-specific monoclonal antibody, whereas the pL(d)-C2 transformants expressed a single 19 kd glycoprotein. A xenoantiserum specific for the exon VI/VIII fusion peptide of H-2D(d) precipitated only the 16 kd product, suggesting that this protein was derived from an alternately spliced transcript lacking exon VII of the native gene. CTL specifically recognized the pL(..